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Time course of release of catecholamines from individual vesicles during exocytosis at adrenal medullary cells.

机译:肾上腺髓质细胞胞吐过程中儿茶酚胺从单个囊泡释放的时间过程。

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摘要

The time course of extrusion of the vesicular contents during exocytosis has been examined at adrenal medullary cells with carbon-fiber microelectrodes. Two electrochemical techniques were used: cyclic voltammetry and amperometry. Spikes obtained by amperometry had a faster time course than those measured by cyclic voltammetry, consistent with the different concentration profiles established by each technique. However, the experimental data obtained with both techniques were temporally broadened with respect to dispersion of an instantaneous point source by diffusion. Measurements with the electrode firmly pressed against the cell surface established that the temporal broadening is a result of a rate-limiting kinetic step associated with extrusion of the vesicular contents at the cell surface. The data do not support a rate-limiting process due to restricted efflux from a small pore. When combined with previous results, the data suggest that the rate-limiting step for chemical secretion from adrenal medullary cells during exocytosis is the dissociation of catecholamines from the vesicular matrix at the surface of the cell.
机译:用碳纤维微电极在肾上腺髓质细胞中检查了胞吐过程中囊泡内容物挤出的时间过程。使用了两种电化学技术:循环伏安法和安培法。通过安培法获得的尖峰比通过循环伏安法测量的尖峰具有更快的时间进程,这与每种技术建立的不同浓度曲线相一致。但是,相对于通过扩散的瞬时点源的分散,通过两种技术获得的实验数据在时间上有所扩大。用电极牢牢地压在细胞表面上的测量结果表明,时间变宽是与细胞表面囊泡内容物挤出相关的限速动力学步骤的结果。由于来自小孔的流出受限,因此数据不支持速率限制过程。当与先前的结果结合时,数据表明在胞吐过程中肾上腺髓质细胞化学分泌的限速步骤是儿茶酚胺从细胞表面的水泡基质中解离出来。

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